‘Of course, that's about ideal. I seek for a physical comprehension of biological programs inside a base-up way. I believe you might distinguish a few lines of exploration in my team for the time being. The primary concerns chromosome construction: How does one get yourself a metre-extended DNA polymer folded and organised in this type of microscopic cell? We now have developed visualisation approaches that allow us to check how, by way of example, SMC proteins loop DNA (video clip on Youtube) in an effort to Develop up chromosomes.
Our group scientific studies bacterial cell division and it is aiming to constitute a ‘base up biology’ in artificial cells which will autonomously divide. Our major curiosity lies in cell division, chromatin construction, and spatial Manage.
To be a human being, I possess the biblical assignment to examine and use everything in creation for the advantage of my neighbour. I will not expertise any conflict in between faith and science. On the contrary, my religion stimulates me to try and do science, to be open-minded on earth.’
I can hardly visualize how I, to be a geneticist, would manage to study elementary physics, let alone compete at the very best. How did you go about it?
We acquire position-of-care diagnostics test of neglected tropical health conditions in just source-constrained settings, dependant on Crspr/Cas9 detecting of pathogen’s DNA in overall body fluids.
Immediate immobilization of native yeast iso-1 cytochrome c on bare gold: speedy electron relay to redox enzymes and zeptomole protein-film voltammetry
What distinguishes biology from chemistry is these factors alongside one another make up a living method. The key of claimed everyday living, I think, lies inside the interactions, the interplay of All those molecules. The massive dilemma concerning the origin of daily life remains to be unanswered. How did we at any time evolve from very simple molecules in to the incredibly intricate devices we see now? We do not ceesdekker.net know. I need to operate on These types of major, open up issues.’
Nanopores signify a sublime and adaptable Instrument to measure one molecules. We research novel detection modes with reliable-state nanopores and several approaches to evaluate proteins with nanopores. Assignments encompass the subsequent:
2003, demonstrated the main biosensors built away from a carbon nanotube; solved the structure and mechanism of DNA maintenance proteins; and discovery of a different method for fabricating solid-point out nanopores for DNA translocation
It was during this era that Dekker performed investigation on magnetic spin devices and on sound in superconductors and semiconductors.
An integrated microfluidic System for quantifying drug permeation across biomimetic vesicle membranes
1999, initially measurement from the wavefunction of solitary molecular orbitals of carbon nanotubes; and discovery of kink heterojunctions of carbon nanotubes which gave decisive proof for any new Luttinger description of interacting electrons in nanotubes
2005, discovery on the mechanism of DNA uncoiling by topoisomerase enzymes; discovery of extended-vary conformational adjustments in Mre11/DNA maintenance complexes; and very first drive measurements on a DNA molecule in the nanopore
Stable-point out nanopores have proven being a shockingly versatile probe for single-molecule Evaluation of DNA. I'll explain some of our modern findings – particularly DNA knots – in addition to our efforts to increase the capabilities of solid-point out nanopores even further more, in the direction of single-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.